Fruit growing
and viticulture of South Russia

Annotation

The necessity of developing multifactorial models for managing the sustainability of reproductive processes in perennial agrocenoses with the participation of orchard crops and grapes is substantiated. The factors influencing the sustainability and efficiency of the production of fruit products and grapes in the Krasnodar region have been identified. Cognitive models for managing the sustainability of reproductive processes in perennial agrocenoses based on technological processes of elements of the fruit and grape-growing management system have been developed. Models for managing the sustainability of reproductive processes are based on cognitive methods of analysis and digital technologies, taking into account the identified systemic relationships in reproductive processes in functional areas of impact according to criteria of efficiency; biologization; environmental management. Cognitive models have made it possible to identify multifunctional relationships in reproductive processes in functional areas of influence, the optimization of which is the basis for the development of digital technologies. The results of cognitive modeling will be the basis for solving applied problems to increase the productivity of fruit agrocenoses, reduce the level of chemical and anthropogenic load on their elements and optimize the costs of production of industrial products. normative parametric model of the stability of reproductive processes in perennial agrocenoses with the participation of orchard crops and grapes has been developed. The tools for managing biotechnological processes in the elements of agrocenosis are proposed.

Annotation

The article presents the results of agrobiological and physiological-biochemical studies to ensure the stability of grape plantations in unstable weather conditions and anthropogenic intensification of production. Studies in the agroecological conditions of the Krasnodar region on the southern chernozems revealed the most productive norm of loading of grape bushes with shoots for the technical varieties Kurchanskiy, Dmitriy, Vladimir, Granatovyi, Antaris and Alkor, on leached chernozems � for the table seedless variety Centennial Seedless. According to the results of electrophoretic separation of peroxidases in polyacrylamide gel and an increased content of ascorbic acid, increased frost resistance was established in the varieties Kristall, Vostorg, Krasnostop AZOS. The Vostorg and Zarif varieties proved to be more adaptive to the stresses of the summer period in terms of leaf hydration, bound water content, the ratio of chlorophylls and carotenoids, the amount of proline and water-soluble sugars, membrane permeability. The role of physiologically active substances in increasing the adaptive potential of grape plants has been established. The treatment of Merlot vines with methyl jasmonate and epibrassinolide during deep dormancy period, as well as proline, salicylic acid and epibrassinolide during induced dormancy period had a positive effect on the condition of the vine: there was a decrease in electrolyte yield. Sustainable grape cultivation is achieved with the systemic use of the �Bioconcentrate-Z� effluent by the non-root method. Against the background of nonroot treatments of Merlot and Cabernet Sauvignon varieties, a higher level of plant water supply, an increase in potassium content, green pigments, organic acids and grape yields were established. Physiologically active compounds and the preparation Turinbash have a positive effect in intensive own-root plantations of native and promising introduced grape varieties against the background of phylloxera infection in the southern part of Dagestan. The created yield forecasting method based on the NDVI of the grape plant and the actual data of multispectral images of the phenophases of flowering and growth allows to increase the accuracy of the calculated yield capacity to the actual one with a deviation from 0 to 0.8 kg per bush, the accuracy of the forecast increases by 2 times by the end of the growth phase and by the beginning of the maturation phase.

Annotation

The study presents the results of a comprehensive phenotypic and genotypic assessment of the gene pool of orchard crops, grapes and breeding of new generation varieties. Taking into account changing weather and climatic conditions, intensification of technologies for cultivating orchard crops and grapes, the need to change the focus of breeding work is becoming more urgent, in terms of increasing the adaptability of varieties. The purpose of the research was to mobilize, preserve, study the gene pool, identify genes for valuable traits using DNA markers, and create varieties with high productive potential. In conjunction with this, the NCFSCHVW preserves a gene pool of 7,240 genotypes, 632 donors and sources of valuable traits. For the first time, promising DNA-markers Ps12a02a and CPPCT006, priority ones for genetic analysis, have been identified for the evaluation of sweet cherry varieties. The polymorphism of DNA-markers used for genotyping of grape varieties was established: UDV305 and UDV737, linked to the Rpv3 gene for resistance to downy mildew, GF15-42, SCGF15-02, linked to the Ren3 gene, and CenGen6, linked to the Ren9 gene for resistance to grape powdery mildew. DNA-marking made it possible to identify donor grape varieties Moldova bessemyannaya (resistance to downy mildew by the Rpv3 gene), Kishmish Zaporozhskiy (resistance to powdery mildew by the Ren3 and Ren9 genes); donor of apple tree immunity to scab according to the Rvi6 gene � elite 12/2-21-27. Analysis of the hybrid progeny of strawberries made it possible to identify the donor of large fruit � Nelly variety. 26 unique DNA passports of domestic grape, plum and apple varieties have been developed. Based on a comprehensive assessment of varieties of orchard crops and grapes, 45 sources were established for important characteristics: adaptability, productivity, tolerance to root phylloxera, seedlessness of grapes, productivity in the mother plantings, easy propagation by cuttings, etc. 16 elite forms were obtained with breeding-significant traits, allowing to optimize the assortment of garden crops and grapes. To create stable and productive agrocenoses and ampelocenoses in the conditions of the North Caucasus, 20 varieties with high ecological plasticity and manufacturability are recommended.

Annotation

The article presents the results of studying the enocarpological characteristics of bunches and berries of local and introduced table and wine grape varieties cultivated in the Absheron region. In the course of the studies, it was found that indicators such as bunch weight, stem weight, skin and pulp residue, juice yield, and the amount of solid residue varied over a wide range depending on the grape variety; in particular, large, medium and small bunches were observed. During the research, it was found that such table and technical grape varieties as Tuya-tish, wild form 2, Alphonse Lavallee, Nagshebi, Digah kechimemesi, Gyozel uzum, Ganjavi, Digah gorasy, Ag Kharji, Uzun salkhym, according to enocarpological indicators (bunch weight, stem weight, skin and pulp residue, juice yield, percentage of seeds in the berry, weight of 100 seeds, solid residue) were noticeably different from other varieties. In the studied table grape varieties, the enocarpological parameters varied over wide ranges, the average weight of the bunch was 210.0-974.0 g, the weight of the stem concerning the total weight of the bunch was 0.7-9.6%, the residue of the skin and pulp was 18.0-40.0 %, juice yield � 51.0-72.7 %, seeds � 2.4-10.8 %, amount of solid residue � 27.3-49 %, and weight of 100 seeds � 2.7-14.0 g. In wine grape varieties, these indicators differed from table varieties. They were estimated as follows: bunch weight 178.7-548.0 g, stem weight concerning the total weight of the bunch � 1.8-6.5 %, the skin and pulp residue � 14.7-31.6 %, juice yield � 64.1-76.2 %, seeds � 2.7-7.4 %, amount of solid residue � 23.8-43.0 %. The highest weight of the bunch, stem, remaining skin and pulp, number of seeds, weight of 100 seeds, and the lowest juice yield were noted in table varieties; for wine grape varieties, this ratio was the opposite.

Annotation

Climate is one of the main factors determining the possibility of grape cultivating and influencing its growth and development. Climatic factors have a huge impact on obtaining high-quality products of grape processing. In the process of work, in order to create an informational database, the archival data on the content of sugars and titratable acids in grapes from the vineyards of Crimea were collected and systematized. According to the criteria necessary for this study, we have selected the information on 8 varieties from the Steppe zone for 11 years, 1 variety from the Piedmont zone for 3 years, and 4 varieties from the South Coast zone for 6 years. By collecting and nonlinear interpolating of long-term observations from Crimean weather stations using geoinformation and mathematical modeling methods, the values of agroclimatic factors were calculated for each selected vineyard on the dates of determining the quality indicators of grapes during their ripening at the location points of the vineyards. For this purpose, 10 agroclimatic indicators characterizing the heat supply were selected. Based on the research results, basic principles for creating an informational database of agroecological resources and quality composition of grapes were developed. Informational database can be further modified and extended with various agroclimatic indicators. Subsequently, the accumulated array of database information will be used to identify patterns reflecting the influence of agroecological conditions on the formation of grape quality parameters for various types of production, which will serve as a basis for establishing viticultural and winemaking terroirs.

Annotation

The article discusses techniques for improving the properties of a nutrient substrate used for planting healthy in vitro grape plants. The research was carried out on the basis of the laboratory of biotechnology ARRIV&W � branch of FSBSI FRARC on an interspecific hybrid Krasnostop Carpi. The purpuse of the study is to improve the ways of adapting healthy in vitro plants to non�sterile conditions. With microclonal reproduction of grapes, death most often occurs when transferring test tube plants to non-sterile conditions. The selection of the composition of the soil substrate and the improvement of its water-physical and biological properties helps to reduce the risk of plant death. In our studies, peat, garden soil and river sand (1:1:1) were used as part of the substrate, to which �Aquasin hydrogel� and �Trichoderma Veride� endomycorrhizal preparation containing mycelium and spores of Trichoderma viride fungi, strain 471, were added. The plants were planted in a 500 ml container, had been adapted to non-sterile conditions and had been grown for 90 days on racks of accelerated plant cultivation (RAPC). In the process, the indicators of the preservation of initial plants, their growth, development and spread of the root system were noted. As a result, it can be concluded that the combined use of hydrogel and mycorrhiza ensures the best survival and preservation of plants of the Krasnostop Carpi variety. The mycorrhizal preparation promotes better development of the leaf surface, and the addition of hydrogel ensures better development of the root system, which will provide plants with better survival in open ground conditions of the basic mother plantation.

Annotation

The search for new promising pear varieties with high adaptation and production potential is an important aim of modern breeding for the southern regions of the Russian Federation. In present study, the results of the physiological characteristics of four pear varieties during the summerautumn season 2022-2023 are presented. Pear trees were planted in the territory of the Prikubanskaya fruit growing in the Krasnodar Region. Three varieties Leven, Lyuberskaya, Flamenco of Russian breeding and the American variety Devo were used. The pear leaves were selected three times during the summer months and in September. The content of soluble sugars and total phenols, as well as the activity of antioxidant enzymes � superoxide dismutase and peroxidase � were determined in the plant material. It was established that an increase of the level of the primary and secondary metabolites accumulation and an increase of enzymatic activity by ~20-75 % occurred in the hotter and drier 2023. The content of soluble sugars significantly increased to ~12.0 mg/g fresh weight in the leaves of the varieties Devo and Leven. The accumulation of phenolic compounds increased in all pear varieties by more than 30 % with values exceeding 27,0 mg/g fresh weight. The level of superoxide dismutase activity in 2023 averaged 13.4 unit/mg protein, which is slightly higher than the values of 2022. Peroxidase activity increased 2-4 times with maximum values of more than 8,0 unit/mg protein per min. in the varieties Leven and Lyuberskaya. In conclusion, the data obtained indicate that the drier and hotter conditions of 2023 were not critical for the normal growth and development of the studied pears varieties, but only initiated the activation of the defense antioxidant system in plant cells.

Annotation

The results of 2023 research on the development of methods for managing the stability of fruit agrocenoses with rational land use are presented. The objects of research are orchard agrobiocenoses under various cultivation conditions. The work used methodological approaches and original techniques developed by the research team. Methods and elements of technologies for increasing the resistance of fruit agrocenoses to a complex of factors with biologized approaches for controlling the development of pathogens and pests, rational formation of the crowns of fruit crops of domestic varieties against the background of the application of biostimulants, techniques for rationing high-quality fruit yield, optimized approaches for optimizing plant nutrition and reproducing soil fertility in biocenotic and molecular levels were developed. The obtained results of scientific research of a fundamental and applied nature are associated with the identification of patterns and disclosure of mechanisms for the formation of the productivity of fruit crops in changing cultivation conditions and create a methodological basis for the development of new elements of technologies for managing the productivity of orchard cenoses, systems for monitoring the resource potential of soils to provide the population with quality products while preserving the environment. New knowledge about the patterns of influence of rootstock genotype on the realization of the production potential of various scion-rootstock combinations of the genus Malus Mill. under conditions of different planting densities, will allow, through the use of the most productive scion-rootstock combinations, to significantly increase the productivity of plantings, make the most efficient use of the garden area, and significantly accelerate the onset of marketable fruiting. New knowledge about the patterns of influence of certain limiting parameters of the soil cover on the condition and productivity of scion-rootstock combinations of fruit crops will be used to develop optimal parameters for assessing the horticultural suitability of soils. The identified features of frost resistance of various pear varieties in terms of the degree of stability of their flower buds under changing weather conditions in different phases of the winter-spring period are the basis for optimizing the placement of pear crops in the North Caucasus zone.

Annotation

The publication presents the results of studies to determine the population size of grape thrips Drepanothrips reuteri Uzel and the extent of distribution of on the Don under different meteorological conditions. The studies were carried out in 2021 and 2022 in the conditions of two agrocenoses of Novocherkassk and Nizhnekundryuchensky Branches of the experimental field of the All-Russian Research Ya. I. Potapenko Institute for Viticulture and Winemaking. The subject of the study was the dynamics of the prevalence and harmfulness of grape thrips on the technical grape varieties Tsvetochnyi and Cabernet severnyi, in an uncovered culture. In the context of the widespread distribution of sugescent pests of grapes, the issue of protecting production plantings and improved basic plantings of the mother plant from them is acute, using the method of monitoring studies to signal the population size using flower traps. Therefore, the study of the development features of grape thrips, both in the conditions of the mother plant of basic plants and fruit-bearing vineyards and their influence on the economically valuable traits of grapes, is relevant. Grape thrips is a relatively new species for grape plants in the Rostov region, migrating from abandoned areas with weeds adjacent to the vineyards in search of food. To contain the prevalence of the grape thrips population below the economically significant threshold of harmfulness in agrocenoses, it is necessary to conduct timely phytosanitary monitoring. Under the conditions of two agrocenoses in 2021 and 2022, two generations of grape thrips developed during the growing season: the first at the end of May, the second at the end of June. The intensity of damage to plants of two grape varieties by grape thrips was not significant and did not exceed the economically significant threshold of harmfulness, so it was decided not to carry out chemical treatments. The presence of a stable number of pest individuals suggests that the population may be capable of wider distribution.

Annotation

As known, tomatoes contain a fairly large amount of biologically valuable substances in their composition. Consumers prefer tomatoes in fresh form. However, being a climacteric crop, tomatoes differ during storage and maturation by a high rate of respiration and evaporation of moisture, which leads to a decrease in valuable nutrients, deterioration in quality, over-ripening and rapid aging, which as a result reduces the marketable appearance of products. Traditional methods of processing tomatoes before storage are not always effective enough to maintain the quality of tomato fruits at the proper level, since it is not always possible to store tomatoes at the recommended low temperature, the necessary relative humidity, etc. In order to increase the storage time of tomatoes while maintaining their quality and safety, it is necessary to search for new modern methods of fruit processing before storage. In this regard, an overview of existing traditional technologies, as well as modern and promising techniques and methods for preparing fresh tomatoes for storage, contributing to the reduction of quantitative losses, preserving qualitative characteristics, is carried out. The most promising methods of preliminary preparation of tomatoes for storage, providing a high level of physical-chemical, organoleptic and microbiological indicators that ensure the safety of stored products are: treatment with ethylene biosynthesis inhibitors and ethylene absorbers, ozonation, modified gas atmosphere, electric field and ultrasound treatment, the use of biological products, the use of active packaging, antimicrobial film-forming coatings, etc. The article describes the work of both domestic and foreign researchers.

Annotation

The work shows the results of the influence of the degree of maturity, storage parameters and processing method on the techno-chemical parameters of zucchini. Thus, during the organoleptic assessment of zucchini of different degrees of maturity, stored at a temperature of 8 to 10 ºC for 18 days, it was found that ELF EMF treatment allows maintaining organoleptic quality indicators. At the same time, the average expert assessment score for zucchini of the 1st degree of maturity treated with ELF EMF was the maximum. The maximum weight loss was observed in zucchini of the 1st degree of maturity, the minimum � in the 3rd degree of maturity. When storing zucchini, weight loss depends on the degree of maturity. The minimum weight loss of zucchini without treatment was noted at the 3rd degree of maturity, and the greatest � at the 1st degree of maturity. It has been established that when storing zucchini, ELF EMF treatment can reduce weight loss compared to the control sample for zucchini of the 1st and 2nd degrees of maturity. When studying changes in the biochemical parameters of zucchini depending on the degree of maturity, the method of processing samples and storage conditions, it was found that they decrease over time. During storage, ELF EMF treatment can reduce the loss of soluble solids by 0.2-0.5 %, and when stored in a refrigerator � by 0.2-0.3 %, depending on the degree of maturity. It has also been established that to assess the degree of maturity of zucchini, the iodine-starch method can be used, the essence of which lies in the ability of starch to give a blue-black color in an iodine solution: hydrolysis of starch during the ripening of zucchini proceeds in the direction from the center to the periphery of the seed chamber, which makes it possible to assess the degree of maturity zucchini fruits.

Annotation

The quality of clarification of apple must from various varieties of apples of domestic and foreign breeding, different ripening periods, growing at the Research and Breeding Collection of Genetic Resources of Horticultural Crops, located in the Central Joint Stock Company, Krasnodar, was studied. Sampling of apples was carried out at optimal ripeness from different sides of the tree crown in relation to the cardinal directions. Apple processing was carried out using a crusher-grinder, followed by pressing the pulp on a hydraulic press. The enzyme preparations SQzyme PCL, Grainzyme NL and Fructozyme R were used in the experiments. Apple must was clarified using bentonite (BENTOVIN), plant (pea) and animal (gelatin) protein. As a result of the studies, it was established that self-clarification of the must without the use of enzyme preparations was slow. Slow self-clarification with the formation of voluminous loose and mobile sediments was observed only in certain variants (Ekzotika, Orfey, Liberti, Renet Platona). The addition of enzyme preparations, especially SQzyme PCL, contributed to an increase in must yield by 12-17 % relative to the control. The most �responsive� to the use of enzyme preparations were apples of the varieties Dzhin, Ekzotica, Orfey, Margo, Prikubanskoe, Bagryanets Kubani, Liberti, Renet Platona, forms 12/1-20-16 and 12/1-20-33. With the addition of enzyme preparations in the pulp, the rate of must extraction increased. Gradual clarification was noted in the next 3-4 hours. The greatest increase in must yield was noted during the processing of apple varieties such as Enterprise, Margo, Ketni, Renet Platona � by 12-17 % compared to the control (without the use of enzyme preparations). The use of only one sorbent � bentonite, animal or vegetable protein � did not lead to high-quality clarification of the must at the given dosages. At the same time, complex processing made it possible to significantly improve the quality of clarification and reduce the turbidity of the must. The highest quality clarification of the must, regardless of the apple variety, was obtained by complex treatment of the pulp with enzyme preparations followed by dosing of proteins and bentonite.

Annotation

In this work, an attempt has been made to study the possibility of using carbon dioxide extracts from plant raw materials as an additive to improve the taste and aromatic parameters of a grape-containing drink. Advancing the technology of juices and drink is mainly aimed at improving their stability indicators, preserving and increasing the content of biologically active substances. Considerable attention in the production conditions is paid to the use of preservation agents, especially if this applies to products intended for infant food. In this work, methods of chemical analysis, capillary electrophoresis, gas chromatography using a capillary column were used to assess the general parameters of the component composition of grape juice. Experiments on the modification of grape drink used carbon dioxide extracts produced by OOO �Karavan� (Krasnodar). To compare the component composition of the extracts, the substances were determined by group characteristics. It has been shown that the use of CO2 extracts increases the stability of grape-containing drink. The effect on the taste, color and aroma of grape juices from Aligote, Riesling varieties was investigated at the following doses of vegetable extracts � 0.005 %, 0.01 % and 0.02 %. The main condition for the use of the carbon dioxide extract additive has been determined � it should not change the taste and aromatic characteristics of grape-containing drink. Among the selected plant extracts, only the addition of 0.005 % of horse chestnut or coconut allowed to ensure the stability of the product and did not change its taste characteristic. The use of these carbon dioxide extracts allowed to increase the stability of grape-containing drink within the warranty period and contributed to its clarification.

Annotation

Hibiscus x moscheutos L. is a promising decorative perennial for inclusion in green plantings in the southern regions of Russia. Currently, there is no data available on the most effective methods of propagating hibiscus in the conditions of southern gardening in Russia. Since 2014, FSBSI �North Caucasian Federal Scientific Center of Horticulture, Viticulture, Wine-making� (Krasnodar) has been carrying out work on introduction and variety study of H. x moscheutos. The collection includes 47 varieties. The purpose of this research is to evaluate the potential of vegetative propagation of promising varieties using the herbaceous cuttings method. The study included 18 varieties and hybrids. In the experiment 2 cutting periods were studied: 1st period � spring (II-III decade of May), 2nd period � summer (III decade of June � 1st decade of July (control). It was established that herbaceous cuttings can be carried out in 2 periods � in the II decade of May, with the placement of mother bushes in protected ground, and in the III decade of June, with the preparation of cuttings from mother plants growing in open ground. For most varieties, spring cuttings are preferable. In this case, young plants overwinter better, and the yield of marketable seedlings after 1 year of growing in pots is higher. During wintering of plants of summer cuttings, a high percentage of death was observed, which is associated with the low winter hardiness of young plants. A significant decrease in the yield of marketable seedlings after 1 year of growing in plants of summer cuttings was detected in the following varieties: Berry Awersome, Midnithe Marvell, Plam Flambe, Perfect Storm � a decrease in the yield of seedlings of I grade was observed by almost 2 times in comparison with plants rooted in spring. The best performance in herbaceous cuttings was found in the following varieties: Cherry Cheescake, Cranberry Crush, Peppermint Schnapps, Jazzberry Jam, Summer in Paradise, Krasny Rusanova, Hybrid � 5. These cultivars combine in their genotype adaptability to abiotic stress factors and increased productivity of uterine bushes during cuttings and provide a high yield of marketable seedlings when grown in pots.

Annotation

The results of studying the collection of foreign ornamental varieties of chaenomeles (Chaenomeles Lindl.) of two species are presented: Ch. speciosa (Sweet) Nakai and Ch. japonica (Thunb.) Lindl. et Spach. and two hybrid groups: Ch.×superba (Frahm) Rehder., Ch.× californica W. Clarke ex C. Weber, preserved at the Krymsk EBS � branch of VIR, and in a private collection in the Republic of Adygea. Valuable physiological features were determined: the timing, degree and duration of the flowering period in the conditions of the northwestern piedmont of the Main Caucasian Range. The characteristics of 25 varieties of chaenomeles are given in terms of color, diameter and degree of doubleness of the flower, parameters of the bush, characterizing the decorativeness of its plants, which will expand the range of flowering shrubs for landscaping city parks and squares. Four varieties with the largest double flowers (53 to 65 mm in diameter) were identified: Kinshiden, Scarlet Storm, Mango Storm, Pink Storm, and two varieties with a high degree of doubleness � Pink Storm and Orange Storm, the flowers of which have more than 30 petals. A clear palette of corolla color of the studied varieties was determined: from white to dark red with various shades, including pink and orange. According to the habit of the bush, the varieties are divided into three groups: eight varieties are tall (above 150 cm), three are low-growing (100 cm on average) and the remaining 14 are medium-sized. It has been established that all the studied varieties are suitable for wide use in landscape construction in the Kuban. As sources for targeted breeding programs, varieties with the highest decorative effect � a large double flower of various colors are recommended: Kinshiden, Jet Trail, Wakaba and varieties of the Storm group.

Annotation

Calendula officinalis L. is one of the most common pharmaceutical crops cultivated not only in the Krasnodar region, but also in the south of the Russian Federation as a whole. In addition to its medicinal qualities, a high content of essential oils, as well as phenols and flavonoids, the crop is an excellent alternative to annual floral and ornamental plants in landscaping. In this regard, the purpose of the research was to evaluate seven varieties of Calendula officinalis L. in terms of duration,intensity and timing of the beginning of flowering, the activity of growth processes, size, color, inflorescence structure and the identification of the most adapted to growing conditions varieties that have high decorative qualities. Proposals were made on the directions of using the studied varieties in ornamental gardening and landscaping of the territory. The studies were carried out in 2022-2023 at the experimental site of private household plots in the central zone of the Krasnodar region. As a result of the research, it was found that the studied varieties of calendula had significant differences in a number of morpho-biometric indicators, but turned out to be highly adapted to the growing conditions in the south of the Russian Federation. According to the combination of ornamental qualities, three varieties of calendula officinalis were identified for cultivation in the central zone of the Krasnodar region. The Tutti-Frutti yellow variety is recommended for planting in flower beds due to the bright flowers of the inflorescence and the low growth of the plants. It is advisable to use the Geisha variety for the design of plots in landscape design and for cutting flowers. In garden and park landscaping, attention should be paid to the King's Cup variety, which stands out for a complex of decorative qualities and a long flowering period.